在体膜片钳技术的新进展

New Advance in In vivo Patch Clamp Technique

在体膜片钳是指在整体动物上直接对其中枢神经元进行全细胞膜片钳记录的技术 ,在生理学和药理学研究中具有良好的应用前景 .常规采用的是盲法记录 ,最近出现的可视法记录 ,采用双光子靶向膜片钳 (two photontargetedpatching ,TPTP)技术 ,通过基因操作在动物脑内目标神经元中构建特异表达的荧光标志 ,可以做到对特定神经元亚群的靶向研究 .对这两种方法的原理和操作进行了简单的介绍 .

The process of in vivo patch clamp is to recoding electrophysiological signal of neurons in vivo by whole cell techniques, which is useful approach in physical and pharmacological research. A blind patch-clamp technique in the intact brain is usually adopted. Now a new visual method are reported and termed as two-photon targeted patching, which uses two-photon imaging to guide in vivo whole-cell recordings to individual, genetically labeled cortical neurons, so that subset of neurons are specially focused. Here both two methods were simply reviewed.