摘要
应用免疫组织化学的过氧化酶-抗过氧化酶复合物(PAP)染色方法,对13例隐源性致纤维化性肺泡炎(CFA)病人的肺组织标本,进行Ⅰ型和Ⅲ型胶原的半定量测定。结合11例皮质激素治疗效果,分析其对疾病活动性的诊断意义。结果显示:①病程早期(≤3月者),肺组织中Ⅲ型胶原占优势,后期(≥24月者),以Ⅰ型胶原为主。两组间有显著差异(P=0.05);②激素治疗的11例中,有效6例,其Ⅲ型胶原/Ⅰ型胶原≥1;无效5例,两者之比小于1。两组间差异也有显著意义(P<0.05)。提示Ⅰ型与Ⅲ型胶原的半定量测定,有助于判断病程早晚和指导临床治疗,即具辅助诊断疾病活动性的价值。
The immunohistochemical (PAP) staining method was used for semiquantita-tive detection of collagen type Ⅰand type Ⅲ in biopsy specimens from 13 patients with cryptogenic fibrosing alveolitis (CFA). The relationship of the results to glucocorticoid treatment response in Ⅱ cases was studied.Positive associations were observed between the ratio of type Ⅲ/type Ⅰ collagen, the course of disease (P = 0.05) and also disease activity (P = 0.015 2).It is suggested that the PAP stain method is a useful and easy one for collagen typing, and that the semi-quantitative detection of collagen type Ⅰ and typeⅢ is a good marker of disease activity,
出处
《上海医科大学学报》
CSCD
1993年第3期192-196,共5页
Journal of Fudan University(Medical Science)
关键词
肺泡炎
活动性
胶原
PAP法
eryptogenic flbrosing alveolitis (CFA)
activity
collagen
PAP stain
