应用聚合酶链反应制备HBV DNA生物素探针

THE RAPID PRODUCTION OF BIOTINYLATED HBV DNA PROBE USING THE POLYMERASE CHAIN REACTION

通过聚合酶链反应将Bio-11-duTP掺入扩增的HBV DNA中,制备了长度为120bp的生物素探针,可检测到0.1pg的HBV DNA。PCR际记法简便迅速,特异性强,所用DNA量少且无需分离纯化,其标记探针得率高,活性强。

By including Bio-11-dUTP during polymerase chain reaction (PCR), HBV DNA probe of very high specific activity has been generated (sensitivity of detection: 0.1pg of HBV DNA). The advantages of PCR labeling include (1) labeling with subnanogram amounts of input DNA; (2) rapid production of vector-free probes; (3) easy regulation of both the specific activity and the amount of labeled probe produced.