大鼠胃肠道伤害性刺激引起的中枢神经系统c-fos表达

c-fos EXPRESSION WITHIN CENTRAL NERVOUS SYSTEM OF THE RAT FOLLOWING GASTROINTESTINAL(VISCERAL) NOXIOUS STIMULATION

本文应用Fos免疫组织化学(ABC法)对大鼠胃肠道伤害性刺激后中枢神经系统内的c-fos表达进行了观察,结果表明:(1)多数核团或部位的c-fos表达于伤害性刺激后的30min开始,2h达高峰,4h后逐渐降低,12h基本恢复正常。(2)Fos免疫反应阳性神经元呈双侧性分布,定位于胸髓(Rexed Ⅰ、Ⅱ、Ⅴ和Ⅹ层)、孤束核、延髓腹外侧区、臂旁外侧核、脑桥室周灰质、中脑导水管周围灰质、楔形核、中缝背核、下丘、丘脑(中线核团、背内侧核、腹后内侧核小细胞部)、外侧缰核、内侧膝状体大细胞部、下丘脑(背内侧核、腹内侧核、室周核等)、中央杏仁核、终纹床核、伏核、外侧隔核、梨状区皮质等。本文对以上部位的c-fos表达规律及其意义进行了初步探讨。

In the present study a total of 24 Sprague-Dawley rats were used. The c-fos expression Within the CNS following gastrointestinal (visceral) noxious stimulation were examinod by using Fos immunohistochemical method (ABC method). The results showed: (1) Following the noxious stimulation, the Fos-like immunoreactive (Ⅱ)neurons were present at 30 minutes, peaked at hour 2, declined after hour 4, and returned to normal condition by hour 12. (2) The Fos-LI neurons were observed bilaterally within the following nuclei or sites: spinal cord (thoracic segments)laminae Ⅰ, Ⅱ, Ⅴ and Ⅹ, nucleus tractus solitarius (nTS), ventrolateral medulla (VLM), reticular formation between nTS and VLM, paratrigeminal nucleus, parabrachial nucleus, central gray of pons, periaqueductal gray, cuneiform nucleus, dorsal raphe nucleus, inferior colliculus, central amygdaloid nucleus, bed nucleus of stria terminalis, accumbens nucleus, thalamus (midline nucleus, ventroposterior parvocellular nucleus, dorsomedial nucleus,), lateral habenular nucleus, medial geniculate nucleus (magnocellular part), hypothalamus (periventricular, dorsomedial, ventromedial nucleus), septal nucleus, piriform cortex, anterior olfactory nucleus, et al. The significance of the c-fos expression within CNS was discussed.