摘要
本实验以L-亮氨酸产生菌黄色短杆菌CF-435为出发菌株,制备原生质体,在原生质体形成率和再生率最佳的条件下,利用紫外线、利福平、氯化锂对原生质体进行复合处理,获得再生突变株,从中挑取单独菌落进行摇瓶发酵,筛选出高产菌株UV_3-29,在含有葡萄糖10%的培养基中发酵72h可积累L-亮氨酸26.73mg/ml,比原始菌种产酸量提高26%.
Protoplast from Brevibacterium flavum flavum CF-435 was treated with UV combined with rifampin and LiGl in optimal conditions for protoplast preparation and regeneration. Strain UV3-29 was obtained after shaking culture of regenerated mutants in a medium containing 10% of glucose for 72 hrs. 26.73mg/ml of L-leucine was produced which is an increase of 26% with the original as 100%.
