锈寄生菌抑制落叶松褐锈病的机理研究

Studies on Disease inhibition mechanism of Larch Brown Rust with a Rust Parasite Sphaerelopsis filum

调查和镜检验证了落叶松褐锈病菌被寄生菌重寄生的前、中、后期即其衰亡至消逝的过程:并进行了病理解剖和摄像;锈寄生菌纯培养株系对枯草杆菌G(+)(Bacillus subtilis)和玉米小斑病菌(Cochliobolus heterostrophoes)均有拮抗作用:该菌发酵液的培养条件以PDA+大豆煮汁的液体培养基、28℃、暗光下、120转/min的电动摇床上、培养5d时该生产菌的发酵液性能最佳;以原发酵液浓缩至5倍的菌液为样品、不接菌的空白培养液为对照,进行高效液相色谱法测定,各25μL分别注入665型、检测器波长为A254nm的液相色谱仪内,样品于5′50″,12′2″,14′45″,显现三个特征峰。

Field survey and microscopic photograph examination in laboratory were proceeded during the beginning, middle and late stage in parasitic process of Triphragmiopsis laricinum and Sphaerellopsis filum. Antagonistic experiments showed that the Sphaerellopsis filum inhibits Bacillus subtilis, Cochliololus heterostrophoes, but not inhibit Fusarium moniliforme, Sclerotinia sclerotiorum, Pallicularia sasakii, Sclerotium rolfsii, S. sp., Pyrenochaeta sp., Pestalotia fungenea, Saccharomyces cerevisiae, Staphylococcus aureus and Escherichia coli. Crude antagonistic substances from Sphaerellopsis filum were monitored by HPLC chromatography. Three characteristic peaks are presented in the HPLC chromatogram from products of the Sphaerellopsis filum.