侧脑室注射雨蛙肽对应激性胃粘膜损伤的影响

EFFECT OF INTRACEREBROVENTRICULAR INJECTION OF CAERULEIN ON STRESS-INDUCED GASTRIC MUCOSAL LESION IN RATS

本工作研究了向侧脑室注射雨蛙肽对束缚四肢再浸水引起大鼠应激性胃粘膜损伤的影响及机制。侧脑室注射雨蛙肽(1.0ng/rat)可显著减轻胃粘膜损伤,抑制胃酸分泌,促进胃壁结合粘液分泌并使胃液中PGE_2含量增加。电镜观察可见胃壁细胞分泌增强。预先侧脑室注射纳洛酮或皮下注射消炎痛可消除雨蛙肽抗胃粘膜损伤和抑制胃酸分泌的效应,但对胃壁结合粘液分泌无影响。侧脑室注射阿托品、酚妥拉明、心得安不影响雨蛙肽的抗损伤作用。上述结果提示:注射到侧脑室的雨蛙肽的抗胃粘膜损伤作用,部分是通过中枢的吗啡受体和促进内源性PGE_2合成而实现的。

The effect of caerulein injected into cerebroventricle on the stress--induced gastricmucosal lesion of rats with tied four limbs immersed in water was investigated.Caerulein (1.0 ng/rat) reduced significantly gastric mucosal lesion, decreased gastricacid content, but increased the gastric content of the mucus and PGE_2. Under electromi-croscope, morphological sign of hyposecretion in the porietal cells and hypersecretion inthe mucus cells could be seen. The effect of caerulein could be prevented by intraven-tricular injection of naloxone or subcutaneous injection of indomethacin, but not by at-ropine, phentolamine and propranolol. The results indicate that the protective effect ofcaerulein on the gastric mucosa is mediated partly by central morphine receptors andpertly by enhancement of endogenous PGE_2.