摘要
在清醒大鼠研究延髓迷走背核复合区(DVC)微量注射五肽胃泌素(G5)对胃窦运动的作用。用慢性植入胃腔外应力传感器记录胃窦运动。单侧DVC内注入G5 0.0025,0.005,0.01μg可明显刺激胃窦的运动,并有一定的剂量相关性,反应发生迅速,时间持续长。外周静脉注入中枢量G5无兴奋胃运动效应。DVC内注G5前20min预先注入抗胃泌素血清(1:100)可取消G5兴奋胃窦运动效应。静脉灌流阿托品(100μg·kg^(-1)·h^(-1))和切断膈下迷走神经可阻断DVC注G5兴奋胃窦运动的作用。DVC注G5对肝门静脉血浆胃动素含量没有影响。研究结果表明,DVC是G5兴奋胃窦运动的脑内作用部位。G5兴奋胃运动的中枢效应是经由迷走胆碱能神经传出的。
Changes in antral motility induced by pentagastrin (G5) microinjected into the dor-sal vagal complex (DVC) were investigated in fasting conscious rats. Antral motilitywas monitored by using chronically implanted force transducer sututed to the antrum.DVC injection of G5 markedly stimulated the amplitude of gastric contraction in a dose-dependent manner. The effect, being rapid in onset and long lasting, could not be mim-icked by intravenous administration of G5 but could be markedly inhibited by microin-jection of antigastrin serum into DVC. Atropine (100 μg·kg^(-1)·h^(-1)) and vagotomycould also abolish the G5 induced antral motility. No significant changes in plasmamotilin were observed in portal venous blood after administration of G5 in DVC. Theseresults suggesed that DVC is an important site of action for G5 induced stimulation ofgastric contraction as mediated by vagal cholinergic pathway.
出处
《生理学报》
CAS
CSCD
北大核心
1993年第3期279-285,共7页
Acta Physiologica Sinica
基金
国家自然科学基金(No.3880347)
关键词
胃运动
五肽胃泌素
迷走神经
dorsal vagal complex (DVC)
gastric contraction
pentagastrin (G5)
motilin
vagus
