摘要
为进一步研究纤溶酶原激活因子(PA)在排卵中的作用,我们观察了促乳素(PRL)对hCG诱导小鼠卵巢PA增加和排卵的影响。实验结果表明;(1)PRL抑制促性腺激素诱导小鼠排卵。当bCG注射18 h后,在输卵管中发现卵子平均为31.1±6.7,而hCG加PRL组为19.7±4.9;当hCG注射24 h后,输卵管中发现卵子数为32.3±10.8,hCG加PRL组为20.3±5.4;其抑制率分别为36.5％和37％;(2)PRL对排卵的抑制作用是通过抑制促性腺激素对小鼠颗粒细胞(GC)和膜-间质细胞(TIC)PA分泌的结果;(3)在离体实验中PRL也明显抑制促性腺激素对小鼠GC PA分泌的作用。这些结果进一步证实PA在排卵过程中的重要作用。
We have demonstrated in this study that prolactin inhibits hCG-induced ovulationin PMSG-primed mice. At 18 h after the hormone treatment the number of ova in theoviducts was found to be 31.7±6.7 (mean±SE) in hCG treated group. The numberwas significantly decreased (19.7±4.9) when 100 μg of prolactin was injected simul-taneously. The same effect of PRL on hCG induced ovulation could be also observed at24 h after the hormone treatment; the values reached respectively to 32.31±10.8 and20.3±5.4. Both in vivo and in the experiments further showed that the inhibition ofthe gonadotropin--induced ovulation by PRL is ?ediated through decreasing in the ovar-ian plasminogen activator activity.
出处
《生理学报》
CAS
CSCD
北大核心
1993年第5期510-516,共7页
Acta Physiologica Sinica
基金
中国科学院基础局(No.S852020)
"八五"攻关(No.859180502)基金
关键词
促乳素
纤溶酶原
激活因子
卵巢
prolactin
mouse
granulosa cells
plasminogen activator
ovulation
