试管苗气培生根法的研究及其在工厂化育苗中的应用

A Study on the Rooting Method of Test—tube Plantletby Air-culture and Its Application toIndustrialized Micropropagation

本文提出毛白杨试管苗不需用培养基仅以饱和湿度空气为介质进行生根的新方法,即:气培生根法,从而使传统的组培生根程序得以简化。气培生根无需严格灭菌并能在试管之外完成,生根苗适应外环境能力增强,移栽成活率高。气培生根用于工厂化微繁育苗能显著地提高繁殖系数和生产效率,并能使试管苗的成本下降35％以上。蔗糖溶液和 CO_2对茎段生根有明显地促进作用。

This paprer presented a new rooting method-rooting by air-culture,which proceeded rooting of test-tube plantlets of Populus Tomentosa Carr. with saturated humidity air instead of medium. It may simplify the traditional rooting procedure of plant tissue culture. It needn't exactitude sterilization and can be performed out of test-tube. This kind of rooted plantlets has high adaptability to outside environments. So a high survival rate can be gained when it was transplanted. Aplication this method to industrialized micropopagation can acheive a higher propagation index and production efficency. and reduce the cost of test-tube plantlets by 35 % or more. Sucrose solution and CO2 enrichment have a obviously promoting effect on the rooting of microcuttings.