植物细胞生长与培养液电导率及过氧化物酶活性的关系

Correlation Between Plant Cell Growth and Changes of Conductivity and Peroxidase Activity

通过植物细胞大量培养技术生产次生代谢物已有成功进行工业化生产的先例。我们在九连小檗(Berberis julianae Schneid)悬浮培养细胞生理生化特性的研究中,发现细胞生长与培养液过氧化物酶活性呈平行的关系,与培养液电导率呈相反的镜像关系。

Suspension cultured cells of Glycyrrhiza ralensis Fisch, Silybum merianum (L)Gaertn and Rauwofia verticillate (Lour)Baill were used to study the correlation between cell growth and changes of conductivity and peroxidase activity in cultured media. The results show that cell growth is inversely correlated with conductivity and directly proportional to peroxidase activity. By this method, it is possible to estimate the cell growth in situ during the growth phase for various plant cells in large-scale cultures.