水稻抗s-氨乙基半胱氨酸细胞突变体的筛选

Selection of Rice Mutants Resistant to Inhibition by s-(2-aminoethyl)-L-cystenine

应用水稻花药培养技术,通过对抗s-氨乙基半胱氨酸(AEC)突变体的选择,研究了细胞水平筛选高含量赖氨酸水稻种质的效果。结果表明,AEC可抑制水稻花药(粉)愈伤组织的形成与生长,用0.5mmol/L AEC进行诱导或继代筛选,可获得抗性愈伤组织并再生绿苗,但仅有少数个体自然加倍正常结实,并保持对AEC的抗性。经多代选择与鉴定,获得了丰产优质突变体A-87306,其糙米游离赖氨酸含量0.43%,较对照增加34.38%。初步认为以AEC为外源胁迫因子,离体筛选水稻高赖氨酸含量的细胞突变体是有效可行的。

The highly lysine-containing rice mutants were selected using s-(2-aminoethyl)-1-cystenine (AEC) as the stress factor. AEC significantly inhibited the callus formation and growth from rice anther. The inhibition effect can be partially relieved by Thr. Under induction by 0.5 mmol/L AEC and screening by subculture AEC-resistant callus were selected and green rice plantlets were generated. Some of them are diploid fertile and AEC-resistant. A mutant, A-87306, with high lysine-content and high grain yield was obtained. Its brown rice contains 0.43% of lysine, and 34.38% more than control.