固定化细胞生产D-异抗坏血酸的研究

Immobilization of Pseudomonas putida Cell for Production of 2-keto-D-gluconic Acid

近年来葡萄糖经微生物发酵制备2-酮基-D-葡萄糖酸(2-KDG)正日益受到重视。2-KDG是合成食品抗氧化剂——D-异抗坏血酸(即异维生素C)的重要前体。自1935年以来,国外对2-KDG的产生和发酵进行了广泛的研究。近几年来国内也进行了这方面的研究。

Immobilized cells offer an interesting alternative to the conventional fermentation. The cells of Pseudomonas putida SCB905 were grown in a seed culture medium at 28℃ for 18h, harvested by centrifugation and used for immobilization studies. Among entrapment techniques of alginate-CaCl_2, carrageenan-KCl and chitosan-K_3 [Fe(CN)_6] systems, the Ca-alginate was the best procedure to consider from the points of menchanical strength, maintenance of cells, productivity and operational stability. There is no difference between the morphology of the immobilized cells and that of the free cells under electron microscope. In semi-continuous fermentation, immobilized cells were used over one month.