摘要
应用转化脂介导转化技术成功地将含有潮霉素磷酸转移酶基因的Ｅ．ｃｏｌｉ质粒ｐＣＧＮ１０５５导入到了小麦（徐州２１１）原生质体内，并由此获得了转化愈伤组织。根据Ｈｐｔ抗生及ＤＮＡ分子杂交证实转化频率高达６．０－８．８％，在分化培养基上转化愈伤组织再生出了白化植株，通过Ｓｏｕｔｈｅｒｎ杂交和酶活性检测证明外源Ｈｐｔ基因存在于转化植株（白化苗）内，并进行了有效的表达。
E. coli plasmid pCGN1055 containing hygromycin phosphotransferase (Hpt) gene under control of plant expression promoter was introduced into wheat protoplasts by lipofectin-mediated transformation, and transgenic albino plantlets were obtained, which were confirmed by assay of Hpt activity and Southern blot. The transformation frequencies were 6.0% and 8.8% according to hygromycin resistance and DNA molecular hybridization (Dotblot). The results indicated the foreign gene existed and expressed in transgenic plantlets.
出处
《生物工程学报》
CAS
CSCD
北大核心
1993年第4期320-323,共4页
Chinese Journal of Biotechnology
关键词
小麦
原生质体
转化脂
转基因植物
Wheat (Triticum asetivum L.)
protoplast
Hpt gene
plantlet regeneration
gene expression
