K-562细胞胰岛素受体酪氨酸蛋白激酶活性及内源性底物的研究

Characterization of Insulin Receptor Tyrosine Kinase and Its Endogenous Substrates in K-562 Cells

本文对丁酸钠诱导分化的人白血病细胞株K-562细胞的胰岛素受体酪氨酸蛋白激酶性及细胞内源性底物进行了研究。结果表明,诱导分化后的细胞酪氨酸蛋白激活酶活性降低,胰岛素受体数量减少,酪氨酸蛋白激酶的一底物蛋白在分化后消失。该研究结果及其在该方面的进一步研究有可能为白血病发病机制的阐明以及为临床治疗白血病开辟靳途径提供一些有价值的线索。

In this paper, the tyrosine protein kinase activity of insulin receptor and its endogenous substrates were investigated on the human leukemia cell line K-562 differentiated by sodium butyrate. The results showed that the tyrosine protein kinase activities and number of insulin receptors in differentiated, cells were reduced. An endogenous substrate for tyrosine protein kinase disappeared in differentiated cells. This study provides a powerful clue not only for eliciting the mechanism of cell growth and carcinogenesis but also for the therapy of leukemia.