平菇子实体钙调素的分离纯化及其与猪脑钙调素的生物活性比较

Preparation and Purification of Calmodulin from Pleurotus Ostreatus Hymenophore and Comparison of the Bioactivity with that of Porcine Brain

平菇萃取液经酸性沉淀、热变性、Phenyl-Sepharose CL-4B疏水亲和层析和DEAE-Cellulose 52离子交換层析分冉出了电泳纯的真菌钙调素。在比较钙调素对磷酸二酯酶活化的能力和ELISA实验的免疫亲和力对发现,平菇钙调素与猪脑钙调素的生物活性有较大差异,提示在钙调素定量测定中有必要考虑到标准钙调素与样品钙调素之间的同源性差异。

Mushroom calmodulin (CAM) proved to be electrophoretic homogeneity is obtained in extract of pleurotus ostreatus hymenophore by means of acidic isoelectric point precipitation, heating treatment, Phenyl-Sepharose CL-4B hydrophobic chromatography and DEAE-Cellulose 52 ion exchange chromatography. In comparison with capability to activate phosphodiesterase (PDE) and with immune affinity in ELISA, there is an evident difference between pleurotus ostreatus CaM and porcine brain CaM in bioactivity, which suggested that the homologous difference between control and specimen should be taken into account in quantitative analysis.