正常椎间盘胶原的研究

Study on the Collagen of Normal Intervertebral Disc

腰椎间盘突出症是引起腰腿痛常见的原因。胶原作为椎间盘结构的主要成分,构成椎间盘的纤维框架,其类型与分布直接决定着椎间盘结构的强度和功能的稳定。本文利用溴化氰消化椎间盘胶原产生多肽,借助于梯度层析。SDS-PAGE及光密度定量扫描等对正常人椎间盘胶原进行了研究。结果表明:正常人椎间盘含Ⅰ型及Ⅱ型两种胶原,它们的分布呈明显而特征性的移行性变化:纤维环外层边缘以Ⅰ型胶原为主(83％),由外向内Ⅰ型胶原逐渐移行为Ⅱ型胶原,靠近髓核处以Ⅱ型胶原为主(72％);髓核中心含有Ⅱ型胶原。此为椎间盘的一个结构特性,以满足椎间盘的特殊功能的需要。

Structural degeneration and herniation of lumbar intervertebral disc is one of the most common causes for the low back pain. Collagen provides a fibrosus framework to the disc. Collagen type and distribution directly affect stabilization of the structure and function of intervertebral disc. The disc Collagen was studied With the ideal method of CNBr-digested collagen,chromatography,SDS-PAGE and scanning by using a densitometer. The results showed that the normal human of intervertebral disc contained two genetic types of collagen (Type Ⅰ and Type Ⅱ collagen). Their relative proportion varied gradually and smoothly across annulus fibrosus, the outer edge was mainly type Ⅰ collagen (83％), at and in the innermost region was mainly type Ⅱ collagen (72％). The central nucleus pulposus contained exclusively type Ⅱ collagen. Such specific structural properties may be needed to meet specific function of the intervertebral disc.