摘要
本文运用定点克隆法并结合鸟枪法,对人类鼻咽癌细胞抹CNE2转化基因Tx中一个与转化作用有关的Eco RI片段,进行了核苷酸序列分析。此种克隆方法的运用,大大减少了DNA模板的数量,并加快了测序的进展。将序列分析结果输入美国NCI的CRAY-2型超级电子计算机作进一步分析,以寻找基因库中的同源序列,酶切位点,开放阅读框架(ORF)。在人类DNA基因数据库中没有找到同源序列,从而证实Tx基因是一种新的人类转化基因。计算机的分析还得出了一系列有价值的结论,为进一步深入探讨这个基因在人类鼻咽癌发病学中的作用,提供了重要的资料。这是首次报道的Tx基因核苷酸序列分析结果。
Nasopharyngeal Carcinoma (NPC) is a common tumor in southern China. The molecular events in NPC cell have been studied for a long time.Recently Cao Y et al have reported that a new transforming gene, designated Tx gene,was isolated from human NPC cell line CNE2.The previous data indicated that this gene was different from a number of known oncogenes, and played a role in NPC carcinogenesis. Using Sanger's technique and ^(35)S labeling method, the 2.8kb EcoRI fragment of the Tx gene has been sequenced. The sequencing data has been sent to NCI (USA) computer center for the analysis of nucleic acid and protein sequence to assess putative coding region,restriction sites and transcription start sites. The sequence homologues to known oneogenes, growth factors and growthfactor reoeptors has been assessed.
基金
CMB及国家自然科学基金