摘要
寡糖的对氨基苯甲酸乙酯衍生物,以及它的全乙酰化产物具有紫外吸收基团适合HPLC分离,研究了氨基柱和十八烷基柱对寡糖混合物的分离性能及检测灵敏度。从免疫球蛋白M中分离到的寡糖组分,衍生后用HPLC进一步分离提纯出另一些含量较少的糖链。
A series of oligosacchrides were briefly derivatized the reducing termini with suitable UV-absorbing moiety (ABEE) and then all particular derivatives were applied to peracetylation, both with and without peracety]ation could be purified by HPLC. We compared with different results of resolution and sensitivity on an aminopropyl-bonded silicon column and C_(18) column. Using this experimental strategy a few minor components isolated from IgM were also obtained and detected. Improved sensitivity and resolution are especially useful for trace quantities of natural oligosacchrides.
基金
国家自然科学基金
关键词
寡糖
免疫球蛋白M
高效液相色谱
Oligosacchrides
High Performance Liquid Chromatography
Immunoglobuliu M
