摘要
本文报道了一种从人精液中提纯前列腺特异抗原的方法,本方法仅需Affigel-Blue gel柱和凝胶HPLC二步层析,即可得到均一的PSA纯品。这是一个快速、有效的提纯方法。PSA是前列腺癌的特异肿瘤标志物。
We report a fast efficient method to purify prostate specific antigen(PSA) from human seminal fluid. The procedure only includes two chromatography steps to obtain homogenous PSA, which are Affigel-Blue gel chromatography and gel filtration HPLC. PSA is a specific tumor marker of prostatic cancer.
基金
国家自然科学基金
关键词
前列腺
抗原
分离
提纯
前裂腺肿瘤
Human Prostate Specific Antigen
Affigel-Blue gel
HPLC
Prostate Cancer