摘要
从半夏(Pinellia ternata)块根中,经95％硫酸铵沉淀和固定化猪甲状腺球蛋白柱亲和层析分离得到一种凝集素,称为半夏凝集素(PTL)。凝胶过滤、SDS-PAGE和免疫双扩散鉴定PTL是均一的样品,分子量约44kD,由四个约12kD的亚基组成。PTL主要存在于半夏的块茎中,茎中也有少量存在。
Pinellia ternata lectin (PTL) has been purified from root tuber juice of Pinellia ternata by precipitation with 95% saturation of ammonium sulfate and affinity chromatography on immobilized porcine thyroglobulin. Purified PTL was identified to be homogeneous by gel filtration, SDS-PAGE and double immunodiffusion. Mr of PTL was about 44kD and it was consisted of four subunits. Majority of PTL occured in root tuber, but there was still small amount in stalk.
基金
国家自然科学基金
关键词
半夏
凝集素
植物
分离
提纯
Pinellia ternata lectin (PTL)
Mannan-binding activity
Affinity chromatography
