摘要
测定荷六种小鼠肿瘤S180肉瘤(实体型和腹水型),腹水肝癌(HepA),艾氏腹水瘤(EC),白血病P388和Lewis肺癌的小鼠腹水和血清中唾液酸含量,结果显示血清中唾液酸含量与肿瘤生长、肿瘤类型有关。腹水中唾液酸含量高,推测肿瘤能比正常组织产生更多唾液酸。对四种腹水肿瘤用阴离子交换树脂层析鉴定,发现HepA腹水中葡萄糖代唾液酸(NcuGc)含量明显低于其它三种腹水瘤。还研究了十几种抗癌药物对荷S180和Lewis肺癌小鼠血清中唾液酸含量的影响。发现吗丙嗪(probimane)和顺铂(DDP)能降低荷瘤小鼠血清中唾液酸含量,提示此二药物在肿瘤治疗中更具选择性。
Six murine tumors including ascitic and solid forms of sarcoma S180, HepA, EC, leukemia P888 and Lewis lung carcinoma were employed in this work. The free sialic acids in both blood and ascites were measured in tumor-bearing mice. The results showed that the content of free sialic acids in blood was relevant to tumor growth and tumor type. A high content of sialic acids in ascites was observed, it suggests that the tumor could produce more sialic acids than the normal tissues.All four types of tumor ascites samples mentioned above were separated with an anion exchange chromatography, and an obviously low content of NeuGc analogues in HepA was found. The influence of some antineoplastic agents on the content of sialie acids in blood was investigated in mice bearing either S180 or Lewis lung carcinoma. The different influence on both tumor growth and serum sialic acid levels of mice bearing tumors was found. Probimane and cisplatin were able to decrease the serum sialio acid level.
关键词
唾液酸
抗癌药
肿瘤
药理
Sialic acids
Antineoplastic drug
Tumor growth
Immunosuppressive effect
