摘要
本文将PBR322与HPV重组质粒DNA转化大肠杆菌HB101株,经氨苄青霉素—四环素平板筛选转化成功的菌落,将其扩增、提取质粒DNA,纯化后用限制性内切酶酶切,琼脂糖凝胶电泳区分不同大小的限制性内切片段,并用电洗脱法回收7.9Kb的HPV DNA片段。在高能卤素灯光照下标记光敏生物素制备HPV DNA探针,并检测其特异性和敏感性。结果表明:此探针具有较高敏感性,可达2.5pg;并且具有较强的特异性。
The recombinant plasmid of HPV with PBR322 was transformated and am-plificated in E.coli HB101, and the Plasinids were isolated and Purified. After the digestion with Restriction eiidoruiclease, the 7.9kb fragments of HPV DNA were collected with electroelution method and then labelled with photobiotic as the HPV DNA probe. The results indicated that the probe had a higher sensitivity and specificity .As small as 2.5 pg of HPV DNA could be detected with this probe
出处
《生物技术》
CAS
CSCD
1993年第4期44-46,共3页
Biotechnology
关键词
人乳头瘤病毒
光敏生物素
DNA探针
Human papillomaviruses
Photobiotic labelled
PNA probe
Dot blot
