用脂肪酸自旋标记研究库存血红细胞膜的流动性

INVESTIGATION ON THE FLUIDITY OF HUMAN ERYTHROCYTE MEMBRANES DURING BLOOD PRESERVATION WITH FATTY ACID SPIN LABELS

用两种脂肪酸自旋标记物5—DOXYL和16-DOXYL研究了ACD-B库存血保存期间红细胞膜的流动性及其温度相关性.结果发现:血液保存期间红细胞膜浅层流动性降低,深层流动性增高;红细胞膜浅层相变温度点明显降低,红细胞膜深层则出现两个相变温度点T_1和T_2在血液保存期间T_1和T_2逐渐接近并最后融合成一个相变温度点.

Two fatty acid spin label, 5-Doxyl stearic acid and 16 -Doxyl stearic acid, were used to investigate the fluidity of human erythrocyte membranes and its temperature relevances during a 35-day period of blood preservation in ACD-B anticoagulant. The results showed: during the preservation period, the fluidity of outer erythrocyte membranes decreased while that in the depth increased; the phase transition temperature of outer erythrocyte membranes decreased significantly, in the depth of the erythrocyte membranes two phase transition points, T1 and T2 of lipids were existed the two phase transition points got close to each other during the preservation period. At the end of the 35 -day period the two points fused to form a single point.