付里叶变换偏振红外光谱法研究紫膜LB膜内菌紫质的取向

ORIENTATION OF BACTERIAHRODOPSIN IN PURPLE MEMBRANE LB FILM A POLARIZED INFRARED SPECTROSCOPIC STUDY

用付里叶变换偏振红外法测量了紫膜LB膜内菌紫质的酰胺Ⅰ、Ⅱ和A带的二色性,计算了菌紫质的α-螺旋取向.实验表明,紫膜内菌紫质的α-螺旋轴同膜法线的平均夹角为13°,即与膜平面的平均夹角为77°.

The dichroism of the amide Ⅰ , Ⅱ , and A bands of purple membrane in LB film has been investigated by means of polarized infrared spectroscopy. The degree of orientation of α-helix of bacteriohrodopsin is estimated. The results indicate that in this film. the α-helices of the protein are titled at. on average. 13° with respect to the normal to the plate of the membrane, a reasonable value compared with that reported in literature.