摘要
利用竹红菌乙素自身的荧光特征,在FPR装置上直接测定了乙素在AH细胞内的侧向扩散系数和荧光漂白的恢复率.实验结果表明乙素在AH细胞内的扩散系数D=3.2×10^(-9)cm^2/S^(-1),荧光漂白恢复比率R=97.8%.上述实验说明乙素在细胞膜内与生物大分子之间没有形成共价键形式的结合状态.
The fluorescence photobleaching recovery technique was used to examine the effect of HB on the lateral diffusion and fluorescence recovery rate in the Ascitic Hepatoma(AH) cell membrane and solutions. The results showed that the lateral diffusion D HB in membrane is 3.2× 10-9cm2s-1 and fluorescence recovery rate is near 97.8%; The experiments illustrated that in AH cell membrane HB is in free state.
出处
《生物物理学报》
CAS
CSCD
北大核心
1993年第4期669-672,共4页
Acta Biophysica Sinica
基金
国家自然科学基金资助项目
