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小鼠胚胎移植初试 被引量:8

Primary Study of Embryonic Implantation in Mice
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摘要 目的:确定囊胚期胚胎的最佳种植时期,提高胚胎移植率。方法:20只产过一窝的KM母鼠分为两组作为胚胎移植的假母,比较了2.5d假母和3.5d假母子宫移植的成功率。结果:2.5d的假母囊胚子子宫移值后的妊娠率为10%,3.5d的假母囊胚子宫移植后的妊娠率为60%。结论:假母子宫内膜发育的同步性与囊胚移植后是否种植并生产有着重要的关系。 ve: To determine the optimal time for blastosphere implantation in order to improve the survival rate of embryonic implantation. Methods: 20 female KM mice that had experienced one birth were divided into two groups and used as pseudo-pregnant mice for embryonic implantation. The successful rates of implantation after 2. 5 days and 3.5 days were compared. Result: The pregnant rate after 2.5 days of the implantation was 10% , compared to 60% after 3.5 days. Conclusion: The synchronous development of the endometria in pseudo-pregnant mice plays an important role in the successful blastosphere implantation in mice.
出处 《广州医学院学报》 2001年第4期36-38,共3页 Academic Journal of Guangzhou Medical College
基金 广东省医学科学技术研究基金资项目
关键词 KM小鼠 胚胎移植 同步性 KM mice embryonic implantation synchrony
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参考文献3

  • 1田小利 陈兰英.转基因动物原理、技术与应用[M].长春:吉林科学技术出版社,1994..
  • 2刘丽均,徐平.小鼠桑椹期、囊胚期胚胎移植方法的探讨[J].上海实验动物科学,2000,20(2):109-110. 被引量:3
  • 3Audrey M.Cummings toxicology of early pregnancy,implantation,and uterine function.Reproductive and developmental toxicology[M].Edited by Kenneth S.Korach.397~411.

二级参考文献1

  • 1Hogan B, Constantini F, Lacy E. Manipulating the mouse embryo[M]. Second edition. New York: Cold Spring Harbor Laboratory Press,1994.173-182.

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