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甲苯对塿土脲酶活性影响的实验研究 被引量:14

Effect of Toluene on Urease Activity of Lou Soil
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摘要 通过脲酶受甲苯作用的研究 ,结果表明甲苯对洋刀豆脲酶活性的影响极其微弱 ,但可显著增强土壤的脲酶活性 ,7个土样的增幅为 1 .4 8~ 3 96倍 ,且甲苯在较低浓度和较短处理时间内可完成对土壤脲酶的作用 ,灭菌土壤吸附洋刀豆脲酶后活性变幅小于 3 98% ,这主要是由于甲苯可能将土壤微生物毒害或致死后 ,释放胞内脲酶 ,并将脲酶等固定在土壤有机 无机胶体上所致 .同时揭示出国内外学者研究结果差异的原因可能是土壤 Urease activity affected by toluene studing results showed as follows: Toluene had little action on Jackbean urease activity,but can remarkably increase soil urease activity in lower toluene concentrations and shorter time, the increasing variation was 1.48~3.96 times than without toluene treatment in seven soil samples tested. The variation was less than 3.98% when sterilized soil samples adsorbed the Jackbean urease. It may be because toluene can kill soil microorganism, release urease within cell and subsequently urease fixed into soil organic inorganic colloidal. Different soil pH and microbiology can lead to vary differently for urease activity.
出处 《环境科学》 EI CAS CSCD 北大核心 2001年第6期91-94,共4页 Environmental Science
基金 国家重点基础研究发展规划项目 (G19990 1180 1-3) 中国科学院知识创新工程重要方向项目(KZCX2 40 1)
关键词 实验研究 甲苯 土壤 脲酶活性 LOU土 toluene soil urease activity Lou soil
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参考文献14

  • 1Manunza B, Deiana S, Pintore M etal. The binding mechanism of urea, hydroxamic acid and N-(N-butyl)-phosphoric triamide tothe urease active site. A comparative molecular dynamics study. Soil Biol & Biochem,1999,31: 789~796.
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二级参考文献6

  • 1周礼恺,土壤学报,1993年,20卷,4期,413页
  • 2袁志发,多元统计分析,1993年,133页
  • 3汪远品,贵州农业科学,1989年,6期,27页
  • 4周礼恺,土壤酶学,1987年,142页
  • 5团体著者,土壤微生物研究法,1985年,268页
  • 6团体著者,土壤农化分析,1980年,33页

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