缺氧习服过程中大鼠骨骼肌能量代谢特点研究

Characteristics of glucose metabolism in rat skeletal muscle during hypobaric hypoxia acclimatization

目的 探讨缺氧习服过程中组织物质代谢特点。方法 将大鼠置模拟海拔 5 0 0 0m低压舱内 ,分别于 5、15、30d后取动脉血 ,测定血气、血糖等 ,取双后肢骨骼肌用3 H 脱氧葡萄糖测定葡萄糖摄取率 ,用葡萄糖氧化酶法测定葡萄糖、糖原含量 ,用酶法测定ATP、磷酸肌酸、乳酸水平 ,并以平原动物作为对照组进行对比分析。结果 ATP含量平原组与缺氧组之间以及各缺氧组之间没有统计学差异 (P >0 .0 5 )。磷酸肌酸含量缺氧 5d组显著低于平原对照组 ,缺氧 15、30d组骨骼肌组织磷酸肌酸含量回复至平原水平。缺氧 5d组糖原含量降低 (P <0 .0 1) ,缺氧 15、30d组回升 ,但仍低于平原对照组 (P <0 .0 1)。缺氧 5d组骨骼肌葡萄糖没有显著降低 (P >0 .0 5 ) ,缺氧 15、30d组葡萄糖含量高于平原对照组、缺氧 5d组 (P <0 .0 1) ,缺氧 30d组较缺氧 15d组进一步增加 (P <0 .0 5 )。缺氧 5d组血糖降低 (P <0 .0 1) ,缺氧 15、30d组增高并高于平原对照组 (P <0 .0 5 )。骨骼肌乳酸的含量先增加后回降 ,其在缺氧 15d组骨骼肌中的含量显著低于缺氧 5d组 (P <0 .0 1) ,缺氧 30d组显著低于缺氧 5d组 (P <0 .0 1)。结论 在缺氧习服之后 ,骨骼肌组织能量产生维持在稳定的水平 ,但是组织物质代谢发生调整 ,对葡萄糖利用能力增强。

Objective To investigate the characteristics of energy metabolism in rat skeletal muscle during hypobaric hypoxia acclimatization.Methods Wistar rats were divided into four groups randomly:control group (H0), hypoxic 5d (H5), hypoxic 15d (H15), hypoxic 30d (H30). Animals of H5, 15, 30 group were exposed to hypobaric hypoxia chamber simulating 5000m high altitude for 5d, 15d, 30d respectively, 23h per day. H0 group stayed outside of chamber. The serum level of glucose and the level of glucose, glycogen, lactate, ATP, phosphate ctreatine (PCr) in rat skeletal muscle were assayed by enzyme methods.Results In skeletal muscle, there was no significant difference of content of ATP among H0, H5, H15, H30. The level of phosphate creatine and glycogen of H5, H15 reduced comparing with H0, but increased in H15, H30 compared with H5. Compared with H0, the glucose level of H5 did not reduce significantly, but in H15 and H30 increased. Blood glucose level of H5 reduced, then increased in H15, H30. The content of lactate of skeletal muscle increased in H5 contrast to H0, then reduced in contrast to H5. Conclusion The energy metabolism retained stability during hypoxic acclimatization, utilizing glucose preferentially may be one of the mechanisms in it.