摘要
报道了测定草莓中维生素C的反相高效液相色谱法。样品中的维生索C在zorbax ODs(5μm)反相色谱柱上分离、以0．025mol/LKH_2PO_4—甲醇(70:30)为流动相洗脱、用紫外检测法检测(λ=254 nm)。维生素C在2．5min内得到了良好的分离,检测范围为0~25mg/L。用于实际样品的分析,效果良好。
A method for the determination of vitamin c in strawberry by reversed-phase high performance liquid chromatography has been reported in this paper. Vitamin c was separated on a Zorbax ODS (5μm) reversed-phase column using a solution of 0. 025 mol/L KH2PO4: methanol = 70:30 as the mobile phase and detected by ultraviolet spec-trophotometric detector at 254 nm. Good separation of Vitamin c was achieved in 2. 5 min . the range of detection is 0 -25 mg/L. The method was used for the analysis of actual samples and good results were obtained.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1993年第8期61-63,共3页
Food Science
关键词
草莓
维生素C
色谱法
测定
Reversed-phase High Performance Liquid Chromatography Strawberry Vitamin C0
