叶酸配伍染料木黄酮对神经细胞早期凋亡的影响

STUDY OF THE SYNERGETIC EFFECT OF FOLIC ACID AND GENISTEIN ON THE EARLY STAGE OF PRIMARY CULTURED NEURON APOPTOSIS

目的:观察叶酸和染料木黄酮的单独及联合作用下对神经细胞早期凋亡的影响,并对其可能机制进行探讨。方法:在原代培养的神经细胞中加入环磷酰胺8μg/ml诱导其发生凋亡,在早期凋亡阶段以高、中、低三个浓度的叶酸和染料木黄酮进行单独及联合干预。用流式细胞仪和扫描电镜对凋亡的情况进行观察。结果:1.流式细胞仪检测结果提示:叶酸和染料木黄酮在一定浓度下均可对神经细胞的凋亡产生明显的抑制作用,且在一定的配伍下二者进行联合干预的效果要优于单独干预;2.由扫描电镜对神经细胞早期凋亡阶段所发生膜变化的观察也支持流式细胞仪检测的结果。结论:染料木黄酮可能通过增强叶酸对神经细胞早期凋亡的抑制作用而达到增强叶酸对神经管畸形的预防作用。

Objective: To observe the synergetic effect of folic acid and genistein on apoptosis in the early stage of neuron and its possible mechanism. Methods: Cyclophosphamide was added to primary cultured neurons to induce apoptosis. Three different dosages of folic acid and genistein were used to inhibit apoptosis. The results were observed by flow cytometry and scanning electron microscopy (SEM). Results: 1.Folic acid and genistein might play a role in early stages of neuron apoptosis, and the synergetic effect was demonstrated when folic acid and genistein were administered together. 2. The membrane changes in early stages of neuron apoptosis observed by scanning electron microscope were also in agreement with the flow cytometry data. Conclusion: Genistein might reinforce the protective effect of folic acid on neural tube defects by restraining the apoptosis of the early stage of neuron.