小麦抗条锈病基因Yr2的SSR标记

SSR Marker of Wheat Stripe Rust Resistance Gene Yr2

为了利用SSR技术寻找与小麦抗务锈病基因Yr2紧密连锁的分子标记,以近等基因系Taichung29*6／HeinesⅦ与感病的背景亲本Taichung29构建F2分离群体,进行了F2单株苗期抗条锈病鉴定。抗性分析表明,近等基因系Taichung29*6／HeinesⅦ中的抗条锈病基因是由单基因控制的。进一步用7B染色体上的45对SSR引物对抗、感亲本及171株F2分离群体进行SSR分析,筛选出一个与小麦抗条锈病基因Yr2紧密连锁的SSR标记WMC364-207 bp／201 bp。通过最大似然法计算,该标记与Yr2基因之间的遗传距离为5.6 cM。

This study was to facilitate the effective use of Yr2 gene and Yr2 gene cloning based on tight linkage. Using near isogenic line Taichung 29 * 6/Heines Ⅶ and Susceptible parent Taichung 29, we established F2 segregation population and identified its stripe rust resistance in seeding stage. Genetic analysis suggested that the stripe rust resistance Taichung 29 * 6/Heines Ⅶ was controlled by a single gene. By SSR analysis with 45 pairs of primers located on 7B chromesome in 171 individuals of F2 segregation population and their resistant and susceptible parents, we screened a molecular marker WMC364-207bp/201bp that linked to the stripe rust resistance gene Yr2. Genetic distance among marker WMC364-207bp/201bp and resistance gene Yr2 was calculated as 5. 6 cM.