黄化油菜突变体Cr3529子叶类囊体膜光谱性质研究

Study on the Spectra Properties of Cotyledon Thylakoid Membranes in Chlorophyll -reduced Rapeseed Mutant Cr3529 and its Wild Type

以发育10d的黄化油菜突变体为材料,分析了突变体油菜子叶类囊体膜的色素含量、室温吸收光谱、叶绿素荧光发射和激发光谱以及蛋白内源荧光光谱的变化.数据显示:与野生型相比,突变体油菜子叶类囊体膜的光合色素Chla和Chlb含量均减少,但Chla/b比值升高;突变体油菜子叶类囊体膜叶绿素捕光能力和受激发能力均下降,且较依赖于Chla捕光并将光能激发传递给PS 反应中心;突变体油菜子叶类囊体膜的蛋白内源荧光也明显异于野生型,进一步表明突变体油菜子叶类囊体膜蛋白组成发生了改变.

From 10-day old rapeseed (Brassica napus L.) mutant type Cr3529 and its wild type 3529, the cotyledon thylakoid membranes were respectively prepared and a comparative study on their chlorophyll composition, room temperature absorption spectra, chlorophyll emission and excitation fluorescence spectra and protein influorescence spectra was conducted. The figures showed that lower Chl (Chl a and Chl b) but a much higher Chl a/b ratio was in thylakoid membranes from the mutant type than in those from its wild type. Furthermore, the chlorophyll fluorescence spectra indicated, on contrary to that in wild type, Chl a rather than Chl b in the mutant type is responsible for harvesting light and being excited by light. In addition, composition of proteins on cotyledon thylakoid membranes also changed in the mutant type, suggesting by significant difference in protein influorescence between these two kinds of membranes.