摘要
本研究目的在于分析重度苯中毒患者外周血单个核细胞中T细胞受体删除DNA环 (T cellreceptorexci sionDNAcircle ,TREC)的含量 ,从而了解其初始 (naive)T细胞的含量和胸腺近期输出功能。利用实时定量PCR和TaqMan方法检测 8例重度苯中毒患者和 16例正常人外周血单个核细胞 (PBMNC)中TREC的水平。结果显示正常人中TREC含量为 :6 .6 9± 4 .79 10 0 0PBMNC ;而重度苯中毒患者则为 1.0 3± 0 .4 4 10 0 0PBMNC ,显著低于正常人TREC水平 (P <0 .0 1) ,并持续苯中毒的全过程 ,即使在外周血血象基本恢复正常时 ,TREC水平仍处于较低状态。结论 :重度苯中毒患者胸腺近期输出功能明显低下 ,苯中毒明显损伤T细胞免疫功能。
The aim of the present study was to investigate the level of T-cell receptor excision DNA circles (TREC) in peripheral blood mononuclear cells (PBMNC) of patients with severe benzene poison, thereby to evaluate the content of naive T cells and the recent thymic output function. Quantitative detection of TREC in DNA of PBMNCs from 16 normal individuals and 8 cases with severe benzene poison was preformed by real-time PCR using TaqMan technique. The results showed that TREC level was 6.69±4.79/1 000 PBMNCs in normal individuals, however, significant decrease was shown in patients with severe benzene poison (1.03±0.44/1 000 PBMNCs, P<0.01). The TREC level was persistently low in period of benzene poisoning, even if peripheral blood cell counts were at normal levels. In conclusion, the recent thymic output function is remarkably decreased in patients with severe benzene intoxication, that may obviously damage the T cell immune function.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第1期114-117,共4页
Journal of Experimental Hematology
基金
国家教育部科技重点项目 [教技司 (2 0 0 1) 2 3 5号 0 10 91]
广州市科委科技攻关项目 (2 0 0 1 Z 0 3 7 0 1 3 )
关键词
苯中毒
T细胞受体删除DNA环
胸腺输出功能
benzene intoxication
T cells receptor excision DNA circle
thymic output function
