骨关节炎Ⅰ号预防氢化可的松所致兔软骨细胞损伤作用

Effect of Osteoarthritis FormulaⅠ Against Chondrocyte Lesion Induced by Hydrocortisone in Rabbits

目的 :探讨骨关节炎Ⅰ号预防氢化可的松对软骨细胞的损伤作用。方法 :用 3～ 4周兔关节软骨 ,经胶原酶Ⅱ消化 ,建立软骨细胞体外培养体系 ,经阿尔辛蓝、甲苯胺蓝染色鉴定后 ,在软骨细胞培养体系中分别加入不同浓度的骨关节炎Ⅰ号与氢化可的松的混合液 ,通过倒置显微镜观察软骨细胞形态变化 ,用MTT比色法观察软骨细胞OD值的变化。结果 :1.2 5 ,2 .5mg/ml氢化可的松培养 2 4h的软骨细胞严重损伤或死亡。 1.2 5mg/ml氢化可的松与 15 0mg/ml骨关节炎Ⅰ号混合液培养的软骨细胞 ,以及 2 .5mg/ml氢化可的松与 30 0mg/ml骨关节炎Ⅰ号混合液培养的软骨细胞生长均良好。MTT检测结果与软骨细胞形态观察一致。结论 :骨关节炎Ⅰ号具有预防氢化可的松对软骨细胞的损伤作用。

Objective:To observe the effect of osteoarthritis formulaⅠ against chondrocyte lesion induced by hydrocortisone . Method:The chondrocyte was isolated from cartilage of 3～4 weeks newly born rabbit by means of collagenaseⅡdigestion and identified by staining method of alcian bllie and toluidine blue. Different doses of the mixed liquor of osteoarthritis formulaⅠand hydrocortisone were added to the chondrocyte and incubated . Morphologic changes of chondrocyte were observed through inverted microscope,and the effects of the mixed liquor of osteoarthritis formulaⅠ and hydrocortisone on the proliferation of chondrocyte were monitored by MTT analysis. Result: 1.25 ,2.5 mg/ml hydrocortisone almost made chondrocyte die on cultivation in vitro for 24 h, meanwhile the cell grew well when both the mixed liquor of 1.25 mg/ml hydrocortisone and 150 mg/ml osteoarthritis formulaⅠand the mixed liquor of 2.5 mg/ml hydrocortisone and 300 mg/ml osteoarthritis formulaⅠwere added to the chondrocyte and incubated, respectively. The result of MTT analysis accorded with cartilage cell morphologic observation. Conclusion: Osteoarthritis formulaⅠcould prevent against chondrocyte lesion induced by hydrocortisone.