期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

一种新的亲合素法分离孕妇外周血中的有核红细胞 被引量：3

A lectin-based method for isolation of nucleated red blood cell from maternal blood
下载PDF
导出
摘要 目的　为了分离孕妇外周血中的有核红细胞用于非损伤性产前诊断。方法　采用了一种新的亲合素法 ,用红细胞表面半乳糖基特异的凝集素 (大豆凝集素 )来选择富集孕妇外周血中的有核红细胞。结果　从 11份 2 .5ml孕 14 + ～ 15 +w的孕妇外周血中都分离到了有核红细胞 ,平均每份血样得到有核红细胞 2 .8个。结论　本方法实验成本低 ,操作简单 ,对于非损伤性产前诊断的研究和推广都具有重要的参考价值。 Objective: To isolate nucleated red blood cells (NRBCs) from maternal blood. Methods: A new technique using galactose-specific lectin was adopted, which based on galactose expression on the surface of NRBC. Results: NRBCs were successfully isolated from 11 blood samples of pregnant women at 14 +～15 + gestational weeks. The average of 2.8 NRBCs were obtained from 2.5ml blood sample. Conclusion: A simple and sensitive method was presented for isolation of NRBCs in maternal blood, which may be helpful for clinical application of noninvasive prenatal diagnosis.
作者 唐爱兰 刘国仰 夏晓艳 高春生 刘歌 张俊荣 张学
机构地区 中国医学科学院中国协和医科大学基础医学研究所医学遗传学系 北京市妇产医院 北京医院
出处 《中国优生与遗传杂志》 2005年第1期13-15,共3页 Chinese Journal of Birth Health & Heredity
基金 美国中华医学基金会资助课题
关键词 大豆凝集素 双密度梯度离心 有核红细胞 非损伤性产前诊断 Soybean agglutinin (SBA) Double density gradient centrifugation Nucleated red blood cell (NRBC) Noninvasive prenatal diagnosis
分类号 R714.55 [医药卫生—妇产科学]
  • 相关文献

参考文献15

  • 1Schmorl G. Athogisch-anatomische untersuchungen uber puerperaleklampsie [M]. 1993,Leipzig:vegel.
  • 2Walknowska J, Conte FA, Grumbach MM. Practical and theoretical implications of fetal/maternal lymphocyte transfer [J]. Lancet I, 1969,1119-1122.
  • 3Bianchi DW, Flint AF, Pizzimenti MF, et al. Isolation of fetal DNA from nucleated erythrocytes in maternal blood [J]. Proc Natl Acad Sci USA, 1990, 87:3279.
  • 4Kitagawa M, Sugiura K, Omi H, et al. New technique using galactose-specific lectin for isolation of fetal cells from maternal blood[J]. Prenat Diagn, 2002,22:17-21.
  • 5Miltenyi A, Mǘller W, Weichel W, et al. High gradient magnetic separation with MACS[J].Cytometry,1990,11:231-238.
  • 6Sekizawa A, Farina A, Zhen DK, et al. Improvement of fetal cell recovery from maternal blood: suitable density gradient for FACS separation[J]. Fetal Diagn Ther,1999,14(4):229-233.
  • 7Parano E, Falcidia E, Conrad T, et al. Noninvasive prenatal diagnosis of chromosomal aneuploidies by isolation and analysis of fetal cell from maternal blood[J]. Am J Med Genet, 2001,101:262-267.
  • 8Cheung MC, James D, Kan YW. Prenatal diagnosis of sickle cell anaemia and thalassaemia by analysis of fetal cells in maternal blood[J]. Nat Genet,1996,14:264-268.
  • 9Sekizawa A, Kimura T, Sasaki M, et al. Prenatal diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood[J]. Neurology,1996,46:1350-1353.
  • 10Lim TH, Tan AS, Goh VH. Relationship between gestational age and frequency of fetal trophoblasts and nucleated erythrocytes in maternal peripheral blood [J]. Prenat Diagn,2001,21:14-21.

同被引文献44

  • 1陈汉平,王陶然,徐晓燕,张铭,相文佩,蒋荣珍,马庭元.孕妇外周血胎儿细胞中巨细胞病毒基因的检测及意义[J].中华检验医学杂志,2004,27(12):823-826. 被引量：6
  • 2曾昭书.HLA-B基因多态性在法医学中的应用研究[M].沈阳:中国医科大学,2005.
  • 3GeQ, BaiY, Liu Z, et al. Detection of fetalDNA in maternal plasma by microarray coupled with emulsions PCR [ J]. Clin Chim Acta, 2006, 369 (1) : 82 -88.
  • 4Guetta E, Gutstein- Abo L, BarkaiG. Trophoblasts isolated fromthematernal circulation: in vitro expansion and potential application in non - invasive prenatal diagnosis [ J ]. Histochem cytochem, 2005, 53 (3) : 337 - 339.
  • 5SekizawaA, Purwosunu Y, Matsuoka R, et al. Recent advancesin non - invasive prenatalDNA diagnosis through analysis of maternal blood [ J ].JObstetGynaecolRes, 2007, 33 (6) : 747 - 764.
  • 6BianchiDW, ZickwolfGK, WelGJ, et al. Male fetal progenitorcells persist in maternal blood for as long as 27 years postpartum [ J ]. ProcNatlAcad SciUSA, 1996, 93 : 705 - 708.
  • 7Purwosunu Y, SekizawaA, FarinaA, et al. EnrichmentofNRBCinmatemal blood: amore feasible- method for noninvasive prenataldiagnosis [ J ]. PrenatDiagn, 2006, 26 (6) : 545 - 547.
  • 8ZhongXY, Holzgreve W, Hahn S. Cell -free fetal DNAin the maternal circulation does not stem from the transplacental passage of fetal erythroblasts [ J ]. Mol HumReprod,2002,8 (9) : 864 - 870.
  • 9BianchiDW, ZickwolfGK, Weil GJ, et al. Male fetal progenitor cells persist inmaternal blood for as long as27 years postpartum [ J ]. ProcNailAcad SciUSA, 1996, 93 : 705 - 708.
  • 10Ohashi Y, Miharu N, Honda H, et al. Correlation of fetal DNA andhuman chorionic gonadotropin concentrations in second - trimester maternal serum [J]. Clin Chem,2002,48(2):386 -388.

引证文献3

二级引证文献3

中国优生与遗传杂志
2005年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部