应用改良巴氏染色法对前列腺炎合并不育症患者精液标本的分析

Analysis of semen from infertile men with prostatitis by using the modified Papanicolaou staining

目的 探讨改良巴氏染色法在前列腺炎合并不育症患者精液标本的应用。 方法 前列腺炎合并不育症患者精液 (n =4 0 ,A组 )和男性不育症门诊初诊者精液 (n =4 0 ,B组 )作改良巴氏染色法分析 ,并比较 2组的精子和非精子细胞染色效果。结果 A组的畸形精子率和白细胞数显著高于B组 (P <0 .0 1)。A组高粘稠度精液标本染色后 ,背景易因涂片较厚而出现杂色 ,精子头、颈和尾部的染色效果与B组的没有差别。而且非精子细胞类型的着色效果也未见与B组的有差别。结论 前列腺炎合并不育症患者精液的畸形精子率和白细胞数有较高的发生率。改良巴氏染色法可应用于前列腺炎高粘稠度精液标本的染色分析 ,实际操作应避免涂片较厚而染上背景色。

Objective: To investigate semen samples from infertile men with prostatitis by using the modified Papanicolaou staining method. Methods: Semen samples were obtained from both infertile men with prostatitis ( group A, n=40) and infertile men who firstly visiting infertile clinic (group B, n=40). The modified Papanicolaou staining method was used to analyze the semen samples. Results: The abnormal sperm morphology rate and white blood cell (WBC) rate in group A were more than those in group B (P<0.01). The staining effect was no different on sperm, immature germ cells and WBC between group A and group B. But the background color was obvious when samples with high viscosity from group A. Conclusions: Semen from infertile men with prostatitis would have high abnormal sperm morphology rate and WBC. The modified Papanicolaou staining method can be used in samples with high viscosity. In practical use, the thick smear would be avoided to reduce the background color.