温里药配伍对口服赤芍小鼠血浆中芍药苷的影响

Effect of compatibility with warming-inside drugs on paeoniflorin in mouse plasma

目的建立适用于7种温里药分别与活血药赤芍配伍后芍药苷血药浓度的RP-HPLC检测方法。探讨活血温里复方的药物配伍机制。方法将胡椒、肉桂、小茴香、吴茱萸、花椒、丁香、干姜分别与赤芍配伍后ig小鼠,采用HPLC法测定各复方在小鼠血浆中芍药苷血药浓度。结果胡椒、肉桂、吴茱萸、小茴香、花椒均能不同程度地提高芍药苷的血药浓度(P<0.01),丁香、干姜对芍药苷的血药浓度提高不明显。结论温里药胡椒、肉桂、小茴香、吴茱萸、花椒与活血药赤芍配伍均能提高赤芍主要有效成分芍药苷的血药浓度。

Objective To establish a RP-HPLC method for the determination of the blood concentration of paeoniflorin which was produced by seven kinds of warming-inside drugs (WID) being used with the promoting-blood drugs (PBD) individually and to explore the mechanism of PBD and WID compound prescription. Methods The blood concentration of peaoniflorin in mouse plasma was determined by HPLC after ig seven kinds of WID being compatible with Radix Paeoniae Rubra (RPR) to mice separately. Results Fructus Piperis (FP), Cortex Cinnamoni (CC), Fructus Evodiae (FE), Fructus Foeniculi (FF), and Pericarpium Zanthoxyli (PZ) compound prescription with RPR can increase the blood concentration of paeoniflorin in different degrees (P<0. 01). But Flos Caryophylii (FC) and Rhizoma Zingiberis (RZ) had obscure effect on it. Conclusion FP, CC, FF, FE, and PZ compound prescription with RPR separately can increase the blood concentration of peaoniflorin.