荒漠肉苁蓉的HPLC指纹图谱研究

Chromatographic fingerprint of Cistanche deserticola by HPLC

目的建立荒漠肉苁蓉Cistanchedeserticola的HPLC指纹图谱,评价不同来源的荒漠肉苁蓉药材的内在质量差异。方法采用HPLC梯度洗脱的方法建立指纹图谱,“计算机辅助相似度评价系统”软件进行数据处理,据此对不同来源的药材进行比较分析。结果方法精密度、稳定性、重现性较好;不同来源的药材指纹图谱差异较大。结论不同来源的荒漠肉苁蓉药材的内在质量差异较大,该方法可用于评价肉苁蓉药材的质量。

Objective To establish chromatographic fingerprint of Cistanche deserticola by HPLC and evaluate the difference of inner qualities of samples from different habitats.Methods HPLC gradient elution was applied to establish the chromatographic fingerprint and 'Computer Aimed Similarity Evaluation System' was used in data analysis.Results This chromatographic fingerprint method has good precision, stability, and repeatability; the fingerprints of the samples from different habitats were quite different.Conclusion There are notable differences in inner qualities of the samples from different habitats.This chromatographic fingerprint method can be used to evaluate the quality of C.deserticola.