大麦幼叶细胞分裂状况和愈伤组织诱导间关系的初步研究(简报)

PRELIMINARY STUDY ON THE RELATION BETWEEN THE IN VIVO MITOTIC ACTIVITY AND THE IN VITRO CALLUS INDUCTION IN BARLEY YOUNG LEAVES

禾谷类叶片培养迄今还有一定难度,虽然已有再生植株的报告,但效率并不高,基因型间差异也极大。

Embryos from the mature seeds ofHordeum vulgate L.cv.Zaoshu No.3 we-re allowed to germinate on MS mediumsupplemented with 1% sucrose.Variationof mitotic index(M.I.)in 0—3mm ba-sal region of first leaves was monitoredfor 49—72h,121—144h and 48,72,96,120,144h seedlings,and those in 0.5mmsuccessive segments between 0 and 10mmbasal region of first leaves for 120h seed-lings as well.For inducing callus,the3mm leaf segments from the base of 1stleaves of different position and differentseedling age were cultured in MS mediumsupplemented with 3mg/L 2,4-D and 0.5mg/L ABA.The M.I.peak of 49—72hseedlings appeared at 14∶00 only,andthose of 121—144h seedlings was fl-uctuately.The M.I.of 48 and 120h se-edlings were higher than those of 72,96and 144h seedlings.The M.I.decreasedgradually along the longitudinal directionof the foliar base,up to zero at 10mmaway from the base.The percentage of ca-llus formation was maximum at 0—3mmbasal segments,The percentage of callusformation of leaf basal segments from 120hseedlings was higher than those from 96and 144h seedlings.