摘要
小麦幼胚在附加2,4-D 2 mg/L+KT0.5 mg/L+LH 300 mg/L+3%蔗糖的MS 培养基上诱导出愈伤组织,继代2—3次后,降低2,4-D 的浓度,该愈伤组织约以60%的频率转变为胚性愈伤组织,继而形成不同发育期的体细胞胚。这些体细胞胚是起源于愈伤组织表层或近表层的单个胚性细胞,体细胞胚经球形胚、梨形胚、盾片胚、成熟胚而成再生植株。在胚性细胞内淀粉粒大最积累,淀粉颗粒大而密集。随着胚性细胞的分裂,淀粉逐渐被消耗,到多细胞原胚期,胚体细胞内淀粉粒趋于消失。球形胚期淀粉粒又开始积累,但到成熟胚期,淀粉积累只限于生长点区域,尔后随着胚体萌发,淀粉含量又趋于减少。这一消长动态过程与小麦合子胚发生中淀粉代谢动态基本一致。
Callus were obtained by culturing theimmature embryos of Triticum aestivum L.on MS medium with 2,4-D 2mg/L,KT0.Smg/L,LH 300mg/L and sucrose 3%.When decreasing the level of 2,4-D to0.2mg/L,embryogenic callus were indu-ced by the efficiency of 60% and somaticembryos at different stages appeared.Thesesomatic embryos originated from singleembryogenic cell of callus on surface orsub-surface.They developed into plantthrough globular stage,pear-shaped stage,scutel stage and mature embryo stage.PASreaction reveled the dynamic change of st-arch during somatic embryogenesis.Therewas a peak accumulation of starch in theform of big and crowded grain in the em-bryogenic cells,but they gradully dimini-shed with the division of cell,and whenthe multicellular proembryos were formed,they almost disappeared completely.Ano-ther accumulation of starch was appearedin the globular stage,and regional distri-bution was formed in mature embryos,then they decreased again with the germi-nation of embryo.This process is similarto that of the zygotic embryos.
出处
《实验生物学报》
CSCD
1993年第3期259-267,共9页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
关键词
小麦
组织培养
体细胞胚发生
淀粉
Wheat tissue culture,Somatic embryogenesis.Starch grain.