MDM2的表达与儿童非霍奇金淋巴瘤关系的研究

Relationship between the expression of murine double minute 2 oncogene and non-Hodgkin lymphoma in childhood

目的 探讨儿童非霍奇金淋巴瘤 (non Hodgkinlymphoma ,NHL)癌基因MDM2 (murinedoubleminute 2 )的表达与NHL的关系。方法 用免疫组化S P法 ,检测NHL、对照组患儿病理组织MDM2蛋白的表达 ,用逆转录聚合酶链反应法 (RT PCR)检测NHL、对照组病理组织和NHL外周血单个核细胞MDM2mRNA的表达。结果 ( 1)MDM2蛋白表达率为 6 4 5 % ,MDM2mRNA表达率为6 1 3% ,与对照组比较均有显著意义 (前者P <0 0 5 ,后者P <0 0 1)。 ( 2 )MDM2蛋白表达率与NHL工作分类、细胞源分类、性别、临床分期、结外侵犯位点间差异没有显著性 (P >0 0 5 ) ;与B状态、乳酸脱氢酶增高间差异有显著性 (P <0 0 5 )。MDM2mRNA表达率与工作分类、细胞源分类、性别、临床分期间差异无显著性 (P >0 0 5 ) ;与B状态间差异有显著性 (P <0 0 5 ) ;与结外侵犯部位、LDH增高间差异有非常显著性 (P <0 0 1)。 ( 3)病理组织MDM2mRNA与MDM2蛋白过表达率、外周血MDM2mRNA过表达率间进行比较 ,结果存在关联性 (P >0 0 5 ,kappa =0 6 5 5和 0 5 71) ,病理组织MDM2蛋白过表达率与外周血MDM2mRNA过表达率间差异也存在关联性 (P >0 0 5 ,kappa =0 6 0 9) )。结论( 1)儿童NHL患者MDM2基因的过度表达率较高。 ( 2 )

Objective To investigate the relationship between the expression of murine double minute 2(MDM2) oncogene and non-Hodgkin lymphoma(NHL) in childhood. Methods Thirty-one cases of NHL were enrolled in this study as patient group and 8 cases of lymphadenitis as control group. (1) Immunohistochemistry ultrasensitive S-P assay was used to detect the expression of MDM2 protein in pathological tissues in all cases. Positive cells were dyed yellow or brown in nuclei. MDM2 positive cell was defined as ≥10% of the tumor cells were positive, which was overexpression of MDM2 protein. (2) RT-PCR (reverse trascription-polymerase chain reaction) was performed to value the overexpression of MDM2 mRNA in the pathological tissues and mononuclear cells in peripheral blood. While the ratio of MDM2/β-actin was >16% was defined as overexpression of MDM2 mRNA. Results (1) Rates of overexpression of MDM2 protein and MDM2 mRNA were 64.5% and 61.3%, respectively, which were significantly different as compared to that of control group ( P <0.05 and P <0.01, respectively). (2) The relationship analysis among subgroups in the experiment group showed that the overexpression of MDM2 protein did not correlate with classifications of working formulation, cellular origin, sex, clinical stage and involved extranodal sites ( P >0.05), but significantly correlated with classifications of B status and the increased serum LDH level ( P <0.05). It was shown that the overexpression of MDM2 mRNA did not correlate with classifications of working formulation, cellular origin, sex and clinical stage ( P >0.05), significantly correlated with B status ( P <0.05), and was remarkably significantly correlated with the involved extranodal sites and the increased serum LDH level ( P <0.01). (3) It was demonstrated that the overexpression of MDM2 mRNA in the pathological tissues was similar to the overexpression of MDM2 protein in the pathological tissues and MDM2 mRNA in peripheral blood ( P >0.05, kappa =0.655 and 0.571), and the overexpression of MDM2 protein in the pathological tissues was similar to that of MDM2 mRNA in peripheral blood ( P >0.05, kappa =0.609). Conclusions (1) The rate of MDM2 oncogene overexpression was quite high. (2) The overexpression of MDM2 protein in pathological tissues determined by using immunohistochemistry ultrasensitive S-P assay was similar to that of MDM2 mRNA in pathological tissues detected by using RT-PCR method. Both methods might be used to detect the overexpression of MDM2 oncogene in the cases of childhood NHL. (3) The overexpression of MDM2 oncogene related to the poor status and poor prognosis of patients with childhood NHL.