摘要
目的研究从细茎石斛[Dendrobiummoniliforme(L)Sw]中分离纯化得到的一种相对分子质量为60×103的多糖DMP2a1的化学结构。方法应用紫外、红外、高碘酸氧化、甲基化分析、质谱及1H,13CNMR等化学和光谱方法研究该多糖的结构特征。结果多糖DMP2a1主链由1,4连接的αD吡喃葡萄糖基组成,βD甘露糖以端基糖的形式位于1,4αD吡喃葡萄糖基的O6位上,每6个1,4连接的葡萄糖残基上有1个O6位发生了取代。结论细茎石斛多糖DMP2a1是一新结构多糖,为首次从该植物中分离得到。
OBJECTIVE: To investigate the chemical structure of DMP2a-1, a polysaccharide isolated from Dendrobium moniliforme (L.) Sw, with molecular weight of 6 kDa determined by high performance gel permeation chromatography. METHODS: Ultraviolet spectrum, infrared spectrum, periodate oxidation, methylation analysis, mass spectrometry and (1H, 13C) NMR spectrometry were used to determine the structure of DMP2a-1. RESULTS: DMP2a-1 was composed of 4-substituted α-D-glucopyranosyl residues. Mannopyranosyl and glucopyranosyl side chains were present as terminal residue on the 6-position of 4-linked glucopyranosyl residues. Approximately one equivalent of substituted groups per six 4-linked glucopyranosyl residue was present. CONCLUSION: DMP2a-1 is a new polysaccharide and is isolated for the first time from this medicinal plant.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第12期900-902,共3页
Chinese Pharmaceutical Journal
基金
浙江省自然科学基金资助项目(301560)
关键词
细茎石斛
多糖
结构分析
Gel permeation chromatography
Infrared radiation
Mass spectrometry
Molecular weight
Nuclear magnetic resonance
Plants (botany)
Ultraviolet spectroscopy
