同源异型盒基因DLX4在乳腺肿瘤中的定量表达研究

The detection of homeobox gene DLX4 expression in breast neoplasm by real time PCR

目的 探讨同源异型盒DLX4基因在乳腺癌发生、发展进程中的可能作用。方法 采用实时荧光定量PCR技术检测 82例乳腺肿瘤组织及 2 2例癌旁正常腺体中DLX4mRNA的表达情况。结果 82例肿瘤组织中 ,有 5 3例呈DLX4阳性表达 (6 4 6 3% ) ,而在癌旁正常腺体中则表达很弱(4 2 2 ,18 18% ) ,差异具有显著性 (P <0 0 5 )。DLX4基因的过表达与病理组织学分级有关 (P <0 0 5 ) ,而与癌肿大小、淋巴结转移、病理类型、肿瘤家族史、雌激素受体及孕激素受体状态无关。不同病理组织分级的乳腺癌标本中DLX4基因的初始拷贝数分别为 (5 13± 0 90 )× 10 3,(1 4 1± 0 4 8)× 10 4 和(3 0 1± 0 5 3)× 10 4 拷贝 μgRNA ,随着组织学分级的升高 ,DLX4基因mRNA表达量呈上升趋势 (F =8 4 7,P <0 0 5 )。结论 DLX4基因在乳腺肿瘤中呈过度表达 ,可能在某种程度上参与了乳腺癌变的发生和发展。

Objective To detect the expression of homeobox gene DLX4 mRNA in human breast neoplasm and explore its role on the development of neoplastic transformation Methods DLX4 mRNA expression was detected in 82 breast neoplasm tissues and 22 adjacent normal tissues by using real time fluorescent quantitative polymerase chain reaction (FQ PCR) Results The expression rate of DLX4 in breast neoplasm and adjacent normal breast tissues was 64 63% (53/82) and 18 18% (4/22) respectively, P =0 003 Overexpression of DLX4 in breast neoplasm was correlated with histological grade ( P =0 024) No correlations were found between DLX4 expression and other clinico pathological factors, including tumor size, lymph node metastasis, family history, pathological type, ER and PR status The initial copy numbers of breast cancer specimen in various histological grades were (5 13±0 90)×10 3, (1 41±0 48)×10 4 and (3 01±0 53)×10 4 copies/μg RNA, respectively With the increase of histological grade, an ascending trend was also found in the expression level of DLX4 mRNA ( F =8 47, P <0 05) Conclusion DLX4 gene is abnormally upregulated in breast neoplasm, hence it might play a crucial role in the carcinogenesis of breast cancer