凋亡抑制蛋白Survivin的表达与肝细胞肝癌预后的关系

The evaluation of Survivin protein expression for the prognosis of postoperative patients with hepatocellular carcinoma

目的 评价Survivin、VEGF和MMP 2在肝细胞肝癌中的表达与HCC预后的关系。方法 采用免疫组化链霉素抗生物素 过氧化物酶 (S P)法检测 6 7例肝癌组织、相应癌旁组织及正常肝脏标本中Survivin、VEGF和MMP 2的表达。选择可能对HCC手术切除患者预后产生影响的临床及生物学因素进行量化 ,依次引入COX风险模型。应用Kaplan Meier法计算累计生存率 ,并经Log rank检验。结果 多因素分析 (COX模型 )显示Survivin的表达、VEGF的表达、肝功能Child分级是与预后相关的独立影响因素。各因素均与术后死亡率有正相关。结论 患者的肝功能分级情况、Survivin、VEGF的表达水平是HCC患者手术后生存的独立危险因素。

Objective To evaluate the correlation between the expression of Survivin、VEGF and MMP 2 and the prognosis of patients with hepatocellular carcinoma Methods Expressions of Survivin、 VEGF and MMP 2 were detected by immunohistochemical S P method in 67 postoperative HCC cases Twelve candidate factors associated with long term survival were analyzed by Kaplan Meier Log rank estimation A multivariative Survival analysis of these individual variables was undertaken using the cumulative survival rate by the COX proportional hazards model Results A multivariative analysis determined three independent significant factors influencing overall cumulative survival including Survivin, VEGF, liver function (Child grade) The three prognostic factors predicted an increased risk of death from HCC Conclusion Survivin, VEGF, liver function, particularly the Child Pugh classification are the most significant prognostic factors for HCC patients undergoing hepatic resection