结脑患儿脑脊液中白细胞介素-2受体的检测

Determination of interleukin-2 recepter in CSF in the child patients with tubercular meningitis

研究目的 探讨脑膜炎的免疫特性,为脑膜炎诊断和预后判定寻找一种简便、可靠的新方法。 研究方法 病例对照研究,采用单抗体与多抗双抗体夹心法测定脑脊液中的白细胞介素-2 受体(sIL-2R),对测定结果进行统计学分析。 结果 脑膜炎患者共102例,结脑92例,化脑6例,病脑4例,其脑脊液中sIL-2R水平分别为271.48±95.58u/ml,255.00±143.31u/ml,155.00±105.00u/ml。正常对照30例,其脑脊液中SIL-2R水平为19.25±9.95u/ml,各脑膜炎组与对照组相比均有显著性差异,10例结脑SIL-2R动态检测结果表明,随病情好转,其SIL-2R水平逐渐下降。 结论 脑膜炎患儿脑脊液中sIL-2R含量明显升高。动态检测结脑患儿脑脊液中的sIL-2R含量可作为判断治疗效果的标准之一。

Objective To search for the immunologic characteristic of the patients with moningitis,so that to look.for a simple and convenient, reliable and new method of disgnosis aud prognosis judgement. Methods case-control research.The solub1e interleukin-2 recepter (sIL-2R) in CSF was determined bydouble antibody sandwich method. Results There are 102 cases of meningitis, 92 cases of tubercular meningitis, 6 cases of purulent men-ingitis and 4 cases of virtis meningitis. The levels of sIL-2R in CSF of them are 271.48±95.58u/ml,265.00±143.31u/ml and 155.00±105.00u/ml. There are 30 normal persons in control group, the level ofsIL-2R in their CSF is 19.25±9.95u/ml. Comparing with control group, there is significant difference onsIL-2R level of each meningitis group. The results of dynamic determination in 10 patients with tubercularmeningitis show that the level of rIL-2R is gradually decreased along with the improvement of illness. Conclusions The content of sIL-2R in CSF of the patients with meningitis are obviously increased.Dynamic determination of sIL-2R in CSF of the patients with tubercular meningitic can regard as one ofthe standards judging the therapeutic effect.