柞蚕部分品种及杂交种的RAPD分析

RAPD Analysis on A Portion of Antheraea pernyi

对柞蚕生产上的部分品种及杂交种进行RAPD分析表明 :供试的柞蚕品种未按体色系统聚类 ,而是按品种的来源聚类 ,胶蓝、烟 6、青黄、方山黄 1号、789聚为一类 ,青 6号、选大 1号、沈黄 1号聚为一类 ;来源相同、体色相同的品种间遗传距离小 ,亲缘关系较近。柞蚕亲本及杂交种F1代的RAPD标记为显性标记 ,通过筛选特定的随机引物 ,应用于柞蚕杂种优势的研究是可行的。

Random amplified polymorphismic DNA (RAPD) techniques were applied to assess genetic varations among some oak silkworm races and their hybrids.The results showed:the races in same group were closely related to their geography distribution and not the skin color.The races were classified into 2 genetic groups.One includes Jiaolan,Yan6,Qinghuang,Fangshanhuang No.1,789,which came from Fangshan in Shandong Province,the others were Qing6,Xuanda No.1,Shenhuang No.1 which came from Shenyang.The difference of reciprocal hybrids and their parents was analyzed with RAPD method.The polymorphisms were inherited as dominant markers.These provided some molecular biological basis for heterosis analysis in Antheraea pernyi