PCR-MASA检测胰腺癌十二指肠液中K-ras基因点突变

Detection of K-ras gene point mutation in duodenal juice of pancreatic adenocarcinoma by PCR-MASA

目的 了解胰腺癌十二指肠液中K-ras基因点突变检测的临床价值。方法 采用PCR-MASA(突变特异性等位基因扩增法)检测胰腺癌患者十二指肠液中K-ras基因点突变。结果胰腺癌患者十二指肠液标本中K-ras基因点突变率为17.4％(4／23),而被检测的急慢性胰腺炎、胰岛素瘤、壶腹癌、胆管癌、十二指肠乳头癌及胃癌病人十二指肠液标本均无K-ras基因突变。结论(1)PCR-MASA法简便、特异、敏感,扩增产物只需常规电泳、染色即可观察结果,元需酶切、杂交、放射性和非放射性显影。(2)对十二指肠液检测K-ras基因第12位密码子有无突变,可有助于判断胰腺病变良恶性及胰腺癌的诊断,但其实用价值尚有待进一步验证。

Objective To study the clinical valuation of detecting K-ras gene point mutation in duodenal juice of pancreatic carcinoma. Methods PCR-MASA(mutant allele-specific amplification) was used to study K-ras gene point mutation in duodenal juice specimens of pancreatic adenocarcino-ma. Results The K-ras gene point mutation rate in duodenal juice of pancreatic adenocarcinoma was 17. 4%(4/23). No mutant K-ras gene was noted in specimens of acute pancreatitis,chronic pancreatitis, insulinoma, ampullary carcinoma, bile duct carcinoma, duodenal papillary adenocarcinoma and stomach cancer. Conclusion (1)This approach is rapid, convenient, specific,sensitive and easy to carry out in clinics with routine methods of acrylamidegel electropheresis and ethidium bromide stain. Restriction exzyme digestion, mutation specific oligonucleotide probe hybridization, radioisotopic and non-radioisotopic imaging were not necessary. (2)Detection of K-ras gene mutation in duodenal juice may serve as a practical new method in clinic for distinguishing pancreatic benign masses from malignant ones and making a definitive diagnosis of pancreatic adenocarcinoma. But its practical value needs to be confirmed further.