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人胰腺癌Hedgehog信号转导途径中PTCH基因表达载体的构建 被引量：1

Construction of PTCH gene vector in Hedgehog signal pathway of human pancreatic cancer
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摘要 目的:克隆人胰腺癌Hedgehog信号通路中PTCH基因,构建PTCH基因表达载体。方法:从人胰腺癌细胞株SW1990抽提总RNA,经RT PCR扩增出PTCH基因,经纯化、回收目的基因PTCH,将其插入表达载体PET22b,转化E.coliBL21,构建重组质粒PTCH/PET22b。结果:从人胰腺癌细胞株SW1990克隆出789 bp的PTCH目的片段,成功构建重组质粒PTCH/PET22b。结论:成功构建重组质粒PTCH/PET22b,为PTCH蛋白表达奠定基础。
作者 邵建国 李兆申 屠振兴 高军 龚燕芳 许爱芳 满晓华 金晶
机构地区 第二军医大学长海医院消化内科
出处 《第二军医大学学报》 CAS CSCD 北大核心 2005年第1期101-102,共2页 Academic Journal of Second Military Medical University
关键词 HEDGEHOG PTCH基因 载体构建 克隆
分类号 R735.9 [医药卫生—肿瘤]
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参考文献8

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同被引文献13

  • 1Shao J, Zhang L, Gao J, Li Z, Chen Z. Aberrant expression of PTCH (patched gene) and Smo (smoothened gene) in human pancreatic cancerous tissues and its association with hyperglycemia[J]. Pancreas, 2006,33 : 38 -44.
  • 2Gao J, Li Z,Chen Z, Shao J, Zhang L, Xu G, et al. Antisense Smo under the control of the PTCH1 promoter delivered by an adenoviral vector inhibits the growth of human pancreatic cancer[J]. Gene Ther, 2006,13 : 1587-1594.
  • 3Wang Y, Gao J, Li Z, Jin Z,Gong Y, Man X. Diagnostic value of mucins (MUC1, MUC2 and MUCSAC) expression profile in endoscopic ultrasound-guided fine-needle aspiration specimens of the pancreas[J]. Int J Cancer, 2007,121 : 2716-2722.
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第二军医大学学报
2005年 第1期

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