硒对饮茶型氟中毒大鼠抗氟能力的影响和骨保护作用

Action and antagonistic effects of selenium on fluorosis associated with brick tea

目的探讨硒对饮茶型氟中毒鼠抗氟能力和骨的保护作用。方法将雄性Wistar大鼠按体质量随机分成6组:对照组、氟化钠组、茶氟组、高硒对照组、高硒氟化钠组和高硒茶氟组。对照组饮用自来水,氟化钠组饮用含氟100mg/L自来水,茶氟组饮用含氟100mg/L的砖茶浸出液,高硒组在饲料中加硒2.97mg/kg。3个月后处死实验动物,测定全血谷胱甘肽过氧化物酶(GSH-Px)活力,分离血清测定血氟,检测氟斑牙发生情况,取股骨干骺端进行骨小梁面密度分析。结果高硒氟化钠组血氟同氟化钠组比较明显降低(t=2.12,P<0.05),有统计学意义,同茶氟组比较无统计学意义。茶氟组和氟化钠组不论是否加硒,两组间氟斑牙和骨小梁面密度比较,差异无统计学意义。结论高硒可以降低氟化钠组大鼠的血氟浓度,但不能明显减轻大鼠氟中毒的病理损伤。

Objective To study the protective effects of selenium on fluorosis associated with brick tea. Methods Male wistar rats were randomly divided into six groups according to body weight: control group with normal feed, sodium fluoride group with normal feed, brick tea group with normal feed, control group with high selenium feed, sodium fluoride group with high selenium feed and brick tea group with high selenium feed. The rats in the control group were given tap water, the rats in the sodium fluoride group were given 100 mg/L sodium fluoride in tap water, and the rats in the brick tea group were given extraction solution of brick tea containing 100 mg/L fluoride. The selenium contents were 0.27 mg/kg and 2.97 mg/kg in normal feed and high selenium feed, respectively. The rats were killed after breeding for three months. The activity of glutathione peroxidase in blood was detected, serum fluoride levels were determined, occurrence of fluorotic tooth was examined and the density of bone trabecula in metaphysis of femur was analyzed. Results Selenium could significantly reduce the serum fluoride contents in rats treated by sodium fluoride(t = 2.12,P < 0.05). There was no difference in the occurrence of fluorotic tooth and the density of bone trabecula between the rat groups of brick tea induced fluorosis and sodium fluorides. Conclusions Higher selenium reduces the serum fluoride level in rats drinking sodium fluoride, but not in rats drinking brick tea extraction solution, and does not relieve the pathologic damage of fluoride.